Amount Of Residues Research Articles

Analysis of the Management of Cereal Crop Residues on Farms to Optimise Cattle Feed in the Western Cotton Growing Zone of Burkina Faso

Crop residues are insufficiently valorised by farmers in western Burkina Faso, while cattle face a shortage of forage. The aim of this study was to analyse the management of cereal crop residues in cotton-cereal farming to optimise their use as feed for draught cattle. A sample of 72 cotton farmers was selected from nine villages in the study area by the stratification method and interviewed using a semi-structured questionnaire. The quantities of cereal crop residue biomass collected by farmers were assessed directly in the field. The results showed that maize and sorghum stalks were predominantly used in cattle feed (86.1 and 90.3%), composting (50 and 44.4%), and mulching (26.9 and 25%), respectively. The average quantities of cereal crop residues stored were 0.83, 0.16, and 1.19 tonnes of dry matter (tDM) for crop farmers, livestock breeders, and agro-pastoralists, respectively, with respective fodder balances of -3.99, -29.05, and -16.99 Tropical Cattle Units (TCU) for the use of cattle, or -0.88, -0.38, and -1.81 TCU for the use of draught cattle during the lean season. Concentrated feed use was 0.20, 0.05, and 0.21 kg/cattle/day; respectively. The main factors influencing the quantities of cereal residues stored on the farms were the size of the cultivated areas and the farmers’ capacity for transportation. Provision of transport equipment and storage infrastructure would therefore appear to be a solution that could improve the level of fodder residue storage, particularly for draught oxen.

Evaluation of management practices in rice–wheat cropping system using multicriteria decision-making methods in conservation agriculture

In this study, we employed two multiple criteria decision-making (MCDM) methods, namely the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) and the Analytic Hierarchic Process (AHP), to determine the best management choice for the cultivation of wheat with a regime of conservation agriculture (CA) practices. By combining alternative tillage approaches, such as reduced tillage and zero tillage, with the quantity of crop residues and fertilizer application, we were able to develop the regime of CA practices. The performance of the regimes compared to the conventional ones was then evaluated using conflicting parameters relating to energy use, economics, agronomy, plant protection, and soil science. TOPSIS assigned a grade to each alternative based on how close it was to the ideal solution and how far away it was from the negative ideal solution. However, employing AHP, we determined the weights of each of the main and sub-parameters used for this study using pairwise comparison. With TOPSIS, we found ZERO1 (0% residue + 100% NPK) followed by ZERO4 (50%residue + 100% NPK), and ZERO2 (100% residue + 50% NPK) were the best performing tillage-based alternatives. To best optimize the performance of wheat crops under various CA regimes, TOPSIS assisted the decision-makers in distinguishing the effects of the parameters on the outcome and identifying the potential for maneuvering the weak links. The outcomes of this investigation could be used to improve management techniques for wheat production with CA practices for upscaling among the farmers.

Coconut Waste: Discovering Sustainable Approaches to Advance a Circular Economy

The coconut tree (Cocos nucifera) stands as a pivotal resource in tropical regions, playing a crucial role in both subsistence and economic activities across Asia, the Pacific Islands, and South America. While the harvesting of coconut fruit is essential for producing globally utilized edible products, such as coconut oil, by small owners and large producers around the world in the food, cosmetics, and pharmaceutical industries, concerns have arisen due to the substantial amount of agro-industrial residue generated in this process, posing environmental risks if they are not properly managed. Recognizing the environmental challenges, this paper emphasizes the transformative potential inherent in coconut waste, characterized by its lignocellulosic composition rich in lignin and multifunctional groups. By delving into the historical context of coconut economic exploration and its chemical composition, this review explores the diverse applications of coconut products, focusing on the utilization and processing of residues to generate sustainable products and byproducts. Ultimately, this comprehensive review underscores the significance of repurposing coconut waste, not only to mitigate the environmental impact but also as a valuable contributor to a circular economy, promoting the use of the lignocellulosic biomass in research and bolstering its role as a raw material in the chemical and energy sectors.

Inhibition of Verticillium Wilt in Cotton through the Application of Pseudomonas aeruginosa ZL6 Derived from Fermentation Residue of Kitchen Waste.

To isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas aeruginosa. Bioassay results demonstrated that this strain possessed robust antagonistic abilities, effectively inhibiting V. dahliae spore germination and mycelial growth. Furthermore, P. aeruginosa ZL6 exhibited high temperature resistance (42o C), nitrogen fixation, and phosphorus removal activities. Pot experiments revealed that P. aeruginosa ZL6 fermentation broth treatment achieved a 47.72% biological control effect compared to the control group. Through activity tracking and protein mass spectrometry identification, a neutral metalloproteinase (Nml) was hypothesized as the main virulence factor. The mutant strain ZL6ΔNml exhibited a significant reduction in its ability to inhibit cotton Verticillium wilt compared to the strain P. aeruginosa ZL6. While the inhibitory activities could be partially restored by a complementation of nml gene in the mutant strain ZL6CMΔNml. This research provides a theoretical foundation for the future development and application of biogas residue as biocontrol agents against Verticillium wilt and as biological preservatives for agricultural products. Additionally, this study presents a novel approach for mitigating the substantial amount of biogas residue generated from kitchen waste fermentation.

Effects of Tcte1 knockout on energy chain transportation and spermatogenesis: implications for male infertility

Abstract STUDY QUESTION Is Tcte1 mutation causative for male infertility? SUMMARY ANSWER Our collected data underline the complex and devastating effect of the single-gene mutation on the testicular molecular network, leading to male reproductive failure. WHAT IS KNOWN ALREADY Recent data have revealed mutations in genes related to axonemal dynein arms as causative for morphology and motility abnormalities in spermatozoa of infertile males, including dysplasia of fibrous sheath (DFS) and multiple morphological abnormalities in the sperm flagella (MMAF). The nexin-dynein regulatory complex (N-DRC) coordinates the dynein arm activity and is built from DRC1–DRC7 proteins. DRC5 (TCTE1), one of the N-DRC elements, has been already reported as a candidate for abnormal sperm flagella beating, however, only in a restricted manner with no clear explanation of respective observations. STUDY DESIGN, SIZE, DURATION Using the CRISPR/Cas9 genome editing technique, a mouse Tcte1 gene knockout line was created on the basis of C57Bl/6J strain. The mouse reproductive potential, semen characteristics, testicular gene expression levels, sperm ATP and testis apoptosis level measurements were then assessed, followed by visualization of N-DRC proteins in sperm, and protein modeling in silico. Also, a pilot genomic sequencing study of samples from human infertile males (n = 248) was applied for screening of TCTE1 variants. PARTICIPANTS/MATERIALS, SETTING, METHODS To check the reproductive potential of KO mice, adult animals were crossed for delivery of three litters per caged pair, but for no longer than for 6 months, in various combinations of zygosity. All experiments were performed for wild type (WT, control group), heterozygous Tcte1+/- and homozygous Tcte1-/- male mice. Gross anatomy was performed on testis and epididymis samples, followed by semen analysis. Sequencing of RNA (RNAseq; Illumina) was done for mice testis tissues. STRING interactions were checked for protein-protein interactions, based on changed expression levels of corresponding genes identified in the mouse testis RNAseq experiments. Immunofluorescence in situ staining was performed to detect the N-DRC complex proteins: Tcte1 (Drc5), Drc7, Fbxl13 (Drc6) and Eps8l1 (Drc3), in mouse spermatozoa. To determine the amount of ATP in spermatozoa, the luminescence level was measured. Also, immunofluorescent in situ staining was performed to check the level of apoptosis via caspase 3 visualization on mouse testis samples. DNA from whole blood samples of infertile males (n = 137 with non-obstructive azoospermia or cryptozoospermia, n = 111 samples with a spectrum of oligoasthenoteratozoospermia, including n = 47 with asthenozoospermia) was extracted to perform genomic sequencing (WGS, WES or Sanger). Protein prediction modeling of human identified variants and the exon 3 structure deleted in the mouse knockout was also performed. MAIN RESULTS AND THE ROLE OF CHANCE No progeny at all was found for the homozygous males which were revealed to have oligoasthenoteratozoospermia, while heterozygous animals were fertile but manifested oligozoospermia, suggesting haploinsufficiency. RNA-sequencing of the testicular tissue showed the influence of Tcte1 mutations on the expression pattern of 21 genes responsible for mitochondrial ATP processing, or linked with apoptosis or spermatogenesis. In Tcte1-/- males, the protein was revealed in only residual amounts in the sperm head nucleus, and was not transported to the sperm flagella, as were other N-DRC components. Decreased ATP levels (2.4-fold lower) were found in spermatozoa of homozygous mice, together with disturbed tail: midpiece ratios, leading to abnormal sperm tail beating. Casp3-positive signals (indicating apoptosis) were observed in spermatogonia only, at a similar level in all three mouse genotypes. Mutation screening of human infertile males revealed one novel and five ultra-rare heterogeneous variants (predicted as disease causing) in 6.05% of the patients studied. Protein prediction modeling of identified variants revealed changes in the protein surface charge potential, leading to disruption in helix flexibility or its dynamics, thus suggesting disrupted interactions of TCTE1 with its binding partners located within the axoneme. LARGE SCALE DATA All data generated or analyzed during this study are included in this published article and its supplemental information files. RNAseq data are available in GEO database (https://www.ncbi.nlm.nih.gov/geo/) under the accession number: GSE207805. The results described in the publication are based on whole-genome or exome sequencing data which includes sensitive information in the form of patient specific germline variants. Information regarding such variants must not be shared publicly following European Union legislation, therefore the access to raw data that support findings of this study are available from the corresponding author upon reasonable request. LIMITATIONS, REASONS FOR CAUTION In the study, the in vitro fertilization performance of sperm from homozygous male mice was not checked. WIDER IMPLICATIONS OF THE FINDINGS This study contains novel and comprehensive data concerning the role of TCTE1 in male infertility. The TCTE1 gene is the next one that should be added to the ‘male infertility list’ because of its crucial role in spermatogenesis and proper sperm functioning. STUDY FUNDING/COMPETING INTEREST(S) This work was supported by National Science Centre in Poland, grants no.: 2015/17/B/NZ2/01157 and 2020/37/B/NZ5/00549 (to MK), 2017/26/D/NZ5/00789 (to AM), and HD096723, GM127569-03, NIH SAP #4100085736 PA DoH (to AY). The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

Synthesis of MOF-5/polyethersulfone (PES) mixed matrix membranes for enhancing membrane filtration performance in polyphenol purification

The various apple products industries produce a large amount of apple residue, which is easily fermented, causes environmental pollution, and its disposal cost is high, but is rich in nutrients, such as polyphenols. Polyphenols can be purified to realize high-value deep processing of apple pomace and to promote energy reuse of food waste. In this study, the highly selective purification of polyphenols was achieved by membrane filtration using prepared Metal-organic framework (MOF)-5/PES mixed matrix membranes with apple peels as raw material. The polyethersulfone mixed matrix membrane was loaded with MOF-5 by the phase inversion method, and their structural and physicochemical properties were characterized by scanning electron microscopy (SEM), and X-ray diffraction (XRD). Zeta potential and specific surface area of MOF-5 particles were measured, as well as the water contact angle and anti-fouling properties of the mixed matrix membrane were analyzed. It was confirmed that the membrane loaded with MOF-5 showed better hydrophilicity and mechanical properties compared with the pristine polyether sulfone membrane. Under practical conditions, the increased hydrophilicity could enhance the anti-fouling properties of membranes, which would improve the flux recovery ratio of membranes. In addition, the prepared MOF-5/PES mixed matrix membrane was applied to the purification of polyphenols, showing excellent purification performance of polyphenols. In particular, the purity of polyphenol after membrane filtration could reach 70.45% when the additional amount of MOF-5 was 10%. This research provides a method to prepare MOF-5/PES mixed matrix membranes, which effectively solves the problem of unstable and unsatisfactory purification effect of commercially available membranes, promotes the development of new materials in membrane science, and realizes high-value deep processing and comprehensive resource development of food waste using membrane filtration.

Development of degradable fiber slickwater system and enhanced proppants-carrying mechanism

Slickwater fracturing fluid has become one of the most popular fracturing fluids due to its low frictional resistance and cleanness. However, slickwater has limited proppants-carrying ability because of its low viscosity. It often leads to proppants deposition in the near-wellbore and even sand plug. Simultaneously, it is impossible to transport the proppants to the distal end of the fractures, affecting the hydraulic fracturing effect. In this work, a degradable fiber slickwater fracturing fluid is innovatively constructed to carry proppants better. The adopted fibers have excellent degradability, no formation residues after complete degradation, and low reservoir damage. A series of rheological experiments are conducted to investigate the rheology of the fiber slickwater. Static proppants-carrying experiments and large visual dynamic proppants-carrying equipment simulate the proppants-carrying process in the reservoir to investigate its proppants-carrying performance. The fiber slickwater has the stronger structure than pure slickwater, the zero-shear viscosity can be enhanced by 20-1000 times, and the viscoelasticity is significantly improved. The dynamic proppants-carrying results show that the amount of proppants residue in the near-wellbore is reduced dramatically, and the proppants are laid more uniformly. About 11.8% of the proppants are delivered to the distal end. Finally, scanning electron microscope and electron microscope characterizations are used to reveal the proppants-carrying mechanism. This endeavor will develop a novel proppants-carrying concept for slickwater fracturing fluid.

Alloying synergistic flame retardant effect on PA6 by polyimide containing alkyl hypophosphate structure

A novel flame retardant − bismaleimide polyalkyl phosphinate aluminium (BPPA), containing both imide and phosphinate structures, was designed and synthesized to investigate the intramolecular synergistic flame retardant effect between imide and phosphinate structures and alloying effect of the imide structure with PA6. Based on the flame retardant and mechanical properties of BPPA/PA6 composites, the alloying flame retardant effect of BPPA on PA6 was systematically investigated. The PA6 material had almost no char after burning; however, BPPA can produce a large amount of residue and form a dense char layer during combustion of PA6 matrix, effectively reducing the heat release, while BPPA also improved the mechanical properties of PA6 composites through the alloying effect. The peak heat release rate (pk-HRR) decreased by 55.8 %, and the residue yield increased by 9.2 wt% for 20BPPA/PA6, compared with the PA6. After compounding aluminum diethylphosphinate (ADP) and BPPA, the flame retardancy of the composites can be further improved. The LOI value of the 8ADP/6BPPA/PA6 was increased to 29.8 % and passed the test of UL94 V-0 level. Its pk-HRR and residue yield were similar to that of the 14BPPA/PA6, which indicated that the compounding system of ADP and BPPA can provide excellent charring properties and gas-phase flame retardancy of PA6. Meanwhile, the alloying BPPA with PA6 not only reduced the effect on the tensile strength of ADP on PA6, but also improved the breaking elongation of the composite. In conclusion, it provides a new direction for developing high-performance halogen-free flame retardant polyamides.

[Determination of six halogenated solvent residues in olive oil by headspace gas chromatography].

The residual amount of halogenated solvents in olive oil is an important indicator of its quality. The National Olive Oil Quality Standard GB/T 23347-2021 states that the residual amount of individual halogenated solvents in olive oil should be ≤0.1 mg/kg and that the total residual amount of halogenated solvents should be ≤0.2 mg/kg. COI/T.20/Doc. No. 8-1990, which was published by the International Olive Council, describes the standard method used for the determination of halogenated solvents in olive oil. Unfortunately, this method is cumbersome, has poor repeatability and low automation, and is unsuitable for the detection and analysis of residual halogenated solvents in large quantities of olive oil. At present, no national standard method for determining residual halogenated solvents in olive oil is available in China. Thus, developing simple, efficient, accurate, and stable methods for the determination of residual halogenated solvents in olive oil is imperative. In this paper, a method based on automatic headspace gas chromatography was established for the determination of residual halogenated solvents, namely, chloroform, carbon tetrachloride, 1,1,1-trichloroethane, dibromochloromethane, tetrachloroethylene, and bromoform, in olive oil. The samples were processed as follows. After mixing, 2.00 g (accurate to 0.01 g) of the olive oil sample was added into a 20 mL headspace injection bottle and immediately sealed for headspace gas chromatography analysis. Blank virgin olive oil was used to prepare a standard working solution and the external standard method for quantification. The solvents used in the preparation of halogenated solvent standard intermediates were investigated and methanol was selected as a replacement for N,N-dimethylacetamide to prepare a halogenated solvent standard intermediate owing to its safety. The effects of different injection times (1, 2, 3, 4, 5, 6 s), equilibration temperatures (60, 70, 80, 90, 100, 110, 120 ℃), and equilibration times (4, 5, 8, 10, 20, 30, 40 min) of the headspace sampler on the detection of the residual amounts of the six halogenated solvents were investigated. The optimal injection time and equilibration temperature were 3 s and 90 ℃, respectively. The method demonstrated good analytical performance for the six halogenated solvents when the equilibration time was 30 min. A methodological study was conducted on the optimized method, and the results showed that the six halogenated solvents exhibited good linear relationships in the range of 0.002-0.200 mg/kg, with correlation coefficients of ≥0.9991. The limits of detection (LODs) and quantification (LOQs) of 1,1,1-trichloroethane and bromoform were 0.0006 and 0.002 mg/kg, respectively. The LODs and LOQs of chloroform, carbon tetrachloride, dibromochloromethane, and tetrachloroethylene were 0.0003 and 0.001 mg/kg, respectively. The average recoveries under different spiked levels were 85.53%-115.93%, and the relative standard deviations (n=6) were 1.11%-8.48%. The established method was used to analyze 13 olive oil samples available in the market. Although no halogenated solvents were detected in these samples, a limited number of samples does not represent all olive oils. Hence, monitoring residual halogenated solvents in olive oil remains necessary for its safe consumption. The LOQs of the method for the six halogenated solvents were significantly lower than that of the COI/T.20/Doc. No. 8-1990 standard method (0.02 mg/kg). In addition, the developed method can be conducted under short operation times with high precision and degree of automation as well as good accuracy. Thus, the proposed method is suitable for the determination and analysis of the residues of the six halogenated solvents in large batches of olive oil samples.

Safety evaluation of the food enzyme bacillolysin from the non-genetically modified Bacillus amyloliquefaciens strain AE-NP.

The food enzyme bacillolysin (EC 3.4.24.28) is produced with the non-genetically modified Bacillus amyloliquefaciens strain AE-NP by Amano Enzyme Inc. The production strain meets the requirements for the qualified presumption of safety (QPS) approach to safety assessment. The food enzyme is intended to be used in 14 food manufacturing processes. Since residual amounts of total organic solids (TOS) are removed in three manufacturing processes, dietary exposure was calculated only for the remaining 11 food manufacturing processes in which the food enzyme-TOS is retained. It was estimated to be up to 35.251 mg TOS/kg body weight (bw) per day in European populations. As the production strain qualifies for the QPS approach and no issue of concern arising from the production process of the food enzyme were identified, the Panel considered that no toxicological studies other than the assessment of allergenicity were necessary. A search for the similarity of the amino acid sequence of the food enzyme to known allergens was made and no match was found. The Panel considered that the risk of allergic reactions by dietary exposure cannot be excluded, but the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use.

Nutritional Evaluation of Peanut Chikki Incorporated with Amla Pomace Powder

Amla processing generates a significant amount of amla residue, known as amla pomace, which is a valuable byproduct. Amla pomace is rich in nutrients and can be utilized as a raw material for further processing and value addition. In this study, peanut chikki was developed by adding amla pomace powder as a functional ingredient at different levels (2%, 4%, and 6%) along with peanuts, and compared with a control sample. The chikki with 6% amla pomace (T3) had the highest overall acceptability score (7.85) compared to other variations. The nutrient composition of the most preferred chikki included protein (10.2g), fat (15.6g), dietary fiber (6.5g), carbohydrates (50g), and 26 mg/100g of ascorbic acid. Storage studies showed an increase in moisture, and peroxide value, a decrease in sensory scores and ascorbic acid content over time. However, the microbiological load remained within acceptable limits. This study demonstrates that amla pomace powder can be successfully incorporated into peanut chikki, providing nutritional and health benefits while addressing waste management issues.

ЭНОКАРПОЛОГИЧЕСКОЕ ИССЛЕДОВАНИЕ ГРОЗДЕЙ И ЯГОД НЕКОТОРЫХ АБОРИГЕННЫХ И ИНТРОДУЦИРОВАННЫХ СТОЛОВЫХ И ТЕХНИЧЕСКИХ СОРТОВ ВИНОГРАДА, ВЫРАЩИВАЕМЫХ В УСЛОВИЯХ АБШЕРОНА

The article presents the results of studying the enocarpological characteristics of bunches and berries of local and introduced table and wine grape varieties cultivated in the Absheron region. In the course of the studies, it was found that indicators such as bunch weight, stem weight, skin and pulp residue, juice yield, and the amount of solid residue varied over a wide range depending on the grape variety; in particular, large, medium and small bunches were observed. During the research, it was found that such table and technical grape varieties as Tuya-tish, wild form 2, Alphonse Lavallee, Nagshebi, Digah kechimemesi, Gyozel uzum, Ganjavi, Digah gorasy, Ag Kharji, Uzun salkhym, according to enocarpological indicators (bunch weight, stem weight, skin and pulp residue, juice yield, percentage of seeds in the berry, weight of 100 seeds, solid residue) were noticeably different from other varieties. In the studied table grape varieties, the enocarpological parameters varied over wide ranges, the average weight of the bunch was 210.0-974.0 g, the weight of the stem concerning the total weight of the bunch was 0.7-9.6%, the residue of the skin and pulp was 18.0-40.0 %, juice yield – 51.0-72.7 %, seeds – 2.4-10.8 %, amount of solid residue – 27.3-49 %, and weight of 100 seeds – 2.7-14.0 g. In wine grape varieties, these indicators differed from table varieties. They were estimated as follows: bunch weight 178.7-548.0 g, stem weight concerning the total weight of the bunch – 1.8-6.5 %, the skin and pulp residue – 14.7-31.6 %, juice yield – 64.1-76.2 %, seeds – 2.7-7.4 %, amount of solid residue – 23.8-43.0 %. The highest weight of the bunch, stem, remaining skin and pulp, number of seeds, weight of 100 seeds, and the lowest juice yield were noted in table varieties; for wine grape varieties, this ratio was the opposite. Key words: GRAPE VARIETIES, LOCAL VARIETIES, ENOCARPOLOGICAL INDICATORS, BUNCH, BERRIES

Exploiting Agri-Food Waste as Feed for Tenebrio molitor Larvae Rearing: A Review.

The agri-food industry generates substantial amounts of waste, including by-products and residues. The increasing demand for sustainable and eco-friendly practices in the agri-food sector has sparked an interest in finding alternative uses for such waste materials. One promising approach is the utilization of waste from the agri-food industry as feed for the rearing of mealworms (Tenebrio molitor). Since agri-food waste is rich in proteins, carbohydrates, lipids, and vitamins, as well as other bioactive compounds, all of which are essential for insect growth and development, incorporating such waste into the diet of mealworms promotes sustainable insect production, reducing the economic and environmental problems associated with waste disposal. This practice can also be beneficial for the rearing of mealworms since their nutritional value can also be enhanced. To this end, various waste materials, such as fruit and vegetable peels, spent grains, and food processing residues, have been investigated as potential feed sources, leading to increased mass production, lower cost, and enhanced nutritional value. This review aims to highlight the potential of agri-food waste as a feed source for mealworms, as well as their potential to enhance their nutritional value. Furthermore, the potential applications of mealworms reared on agri-food waste are highlighted, including their potential as a sustainable protein source for human consumption and as feed ingredients in the livestock and aquaculture sectors.

Comparative analysis of antibiotics in broiler meat using different methods

The poultry industry is one of the fastest-growing industries worldwide, and broiler chicken meat is one of the main sources of meat. To speed up the growth of chickens in a short period of time, poultry farmers use antibiotics that prevent disease and stimulate growth by increasing the rate of feed intake and reducing mortality from pathogen attacks. This study may be useful in analyzing antibiotic residues and aid in scientific research. Samples of broiler chicken meat from different producer firms were purchased from the Almaty market. The analysis was done by chromatographic method and enzyme immunoassay. The Ridascreen test kit was used for the immunoassay. This article presents the results of the study of meat of broiler chickens of domestic and foreign producers for the presence of antibiotic residues used as growth stimulants. The researches have shown that imported meat of broiler chickens from the USA has 10-20% higher antibiotic content than the permitted level by the amount of antibiotic residues, and meat from Ukraine is 10-13% higher in antibiotcs than the permitted level. The content of chloramphenicol is within the norm in all samples. Kazakhstan and Russian samples of broiler chicken meat meet the requirements of regulatory documents in force on the territory of the EAEU and the results showed that they contain only traces of antibiotics chloramphenicol and tetracycline. Analysis of imported broiler chicken meat (USA, Ukraine) showed the presence of antibiotic residues such as tetracycline and chloramphenicol, exceeding the maximum allowable level. To find out the effect of heat treatment on reducing the amount of antibiotics in broiler chicken meat, pates were made using different modes of heat treatment. As a result of heat treatment, the antibiotic content of poultry muscle tissue is significantly reduced.

Ecological problems of technogenically disturbed lands on the Absheron Peninsula


 
 
 The study area occupies an important place in our republic’s economic, political, and agricultural development. It is very important to properly use the land of an area with such an advantageous position. In addition to rich deposits of oil, gas, and gas condensate, the Absheron Peninsula has widespread mineral deposits (limestone, gypsum, crushed stone, bentonite, sand, etc.). Currently, the accumulation of waste in the territory of quarries and dumps after the extraction of non-metallic minerals on a large scale has led to the formation of technogenically disturbed areas. On the territory of the quarries themselves and adjacent lands, well-developed soils are widespread (irrigated ordinary gray soils with different granulometric composition and thickness, semi-desert gray-brown soils). During the extraction of deposits, as a result of the use of multiton machines and mechanisms, these lands were subjected to varying degrees of technogenic degradation, lost their fertility, and have not yet been studied either genetically or economically. Therefore, when writing this article, the main goal was a large-scale study of these lands and the development of recommendations for their restoration. Various scientists have conducted research on the geochronology of the formation of the soil and vegetation cover of the Absheron Peninsula, the degree of exposure to technogenic impacts, the development of a typological classification, mapping and modeling them to a fertile state in order to evaluate them in terms of biological productivity. However, it has not been possible to fully study the land and use it in different economic sectors. For this reason, an important topic of our time is touched upon in the presented article and is chosen again due to its relevance. As an object of study, the soil and vegetation cover of the territory where organic and non-metallic resources of the peninsula were mined, as well as lands that remained outside the scope of large-scale research, spread over the deformed areas of the Absheron Peninsula, were chosen. In order to give the research a greater scientific-theoretical and scientific-practical orientation, we laid soil-vegetation sections, described natural vegetation, and took sam- ples for laboratory analysis from reference places in the study area,. According to the analysis in the studied soils, it can be said that the amount of dry residue, which is considered the main indicator of the degree of salinity, ranges from 0.172-2.312% in the upper reaches of the accumulative-humus layers, which indicates that a small part of these soils has a predominantly weak medium, strong and very strong salinity.
 
 

Management of medications disposal and the economic burden in a sustainable Latin American hospital

BackgroundGlobal health systems allocate high budgets for the acquisition of medicines. However, these medicines become large amounts of drug residue upon expiration or damage. This study is the first at the hospital level in Costa Rica to analyze the management and economic impact of medication waste. MethodsA retrospective study was conducted on the disposal of pharmaceutical products and their economic impact in a private hospital with a supply chain program from 2020 to 2022. ResultsThe implementation of a supply chain program led to the incorporation of preventive strategies to reduce waste generation and updated the waste management process As well as updating the waste management process. In the period 2020–2022, most medications discarded were due to expiration. The most discarded medications were from the alimentary tract and metabolism group, followed by those from the cardiovascular system group and the nervous system group. In terms of cost, systemic antimicrobials followed by antineoplastics were the main contributors. The year 2020 had the most waste, while 2021 incurred the highest expenditure associated with the disposal of medicines. ConclusionA supply chain program led by a pharmacist could reduce waste generation and costs associated. While these results provide insight into the state of our hospital, they cannot be extrapolated to the national level since we suspect that the situation could be worse.

Enzymatic impregnation by high hydrostatic pressure for preparing shape-retaining softened broccoli and carrot

This study examined the feasibility of utilizing high-pressure processing (HPP) with enzymatic impregnation to soften vegetables. Freeze–thaw (FT) processing was used as a positive control to compare the hardness, physicochemical characteristics, and cell microstructure of vegetables. Carrot and broccoli samples were subjected to 200 MPa pressure for 15 min and then impregnated using a Viscozyme L enzyme solution. Compared with the hardness of fresh samples, the hardness of the carrot and broccoli samples that underwent HPP and enzymatic impregnation decreased by 84.6% and 95.5%, respectively, and the hardness of the carrot and broccoli samples that underwent FT processed decreased by 73.6% and 91.3%, respectively. In particular, HPP with enzymatic impregnation reduced the content of insoluble dietary fibers (18%), increased the content of soluble dietary fibers (3%), and considerably increased the concentration of carotene (1.9 times) and isothiocyanate (4.5 times) in carrots and broccoli, respectively. Scanning electron microscopy reveals that HPP physically damaged the intercellular compartments of the vegetables, thereby increasing the impregnation of enzymes. In addition, the level of HPP and the holding time increased, and the amount of vegetable residue and the degree of moisture retention substantially decreased, indicating a negative correlation between damage and hardness. Moreover, HPP pretreatment require a short processing time then FT, which promoted the penetration of enzymes and the internal microstructural biodegradation of vegetables, and it also helped these vegetables release more soluble nutrients. Overall, HPP can be used with enzymatic impregnation to obtain texture-modified vegetables for older consumers with mastication difficulties.

A Rapid and Easy Procedure of Enzyme Biosensor based on Nitrogen-Doped Graphene for Detection of Methyl Parathion in CHM

Methyl parathion (MP), an organophosphorus pesticide that is frequently used to control pests during the planting process of Chinese herbal medicine (CHM), has been used without sufficient control, leading to excessive residues on the surface of CHM, which has a serious impact on the quality and safety of CHM and their preparations. Consequently, it is crucial to carefully control MP during the cultivation, processing, and manufacture of CHM. Based on this, nitrogen-doped graphene (N-Gr) with a high conductivity and chitosan (CS) with good stability were used to modify the glassy carbon electrode (GCE). Subsequently, the prussian blue (PB) with catalytic activity and gold nanoparticles (AuNPs) with excellent biocompatibility were deposited by the electrodeposition method to form the AuNPs/PB/CS@N-Gr/GCE. Then, the acetylcholinesterase (AChE) was effectively immobilized on the electrode surface by covalent bonding between AuNPs and AChE through gold-sulfhydryl bonds. Finally, a rapid and easy procedure of enzyme biosensor (AChE/AuNPs/PB/CS@N-Gr/GCE) for sensitive detection of MP pesticide residues was fabricated. Herein, PB catalyzes the redox reaction of thiocholine, which is produced when acetylthiocholine iodide (ATCHI) undergoes efficient hydrolysis catalyzed by AChE. This process effectively promoted electron transfer, amplifying the sensor’s response signal. After the experimental conditions are optimized, the limit of detection (LOD) for MP is found to be 9.47 × 10−5 μg ml−1. Exhibits a good linear relationship within the concentration range of 1 × 10−3 μg ml−1 ∼ 1 × 101 μg ml−1. Significantly, the fabricated enzyme biosensor excels in swiftly and sensitively detecting trace amounts of MP in real examples. Furthermore, it exhibits robust stability and reproducibility. The excellent performance of this enzyme biosensor not only offers a rapid and easy way to identify and find minute amounts of trace MP pesticide residues in CHM, but also serves as a technical guide for the creation of new, portable, and on-site pesticide residue detection technology for law enforcement.

A proposal to reduce the amount of permethrin entering wastewater resultant from topical use to treat scabies

The incidence of scabies is rising in the last years. Subsequently, the use of pharmaceuticals to treat the disease has also increased. Treatment with topical permethrin is usually recommended as a first line agent.This substance is also an aquatic contaminant that is toxic for many non-target organisms, and has been included as a priority substance in the recently published proposal of the European Water Framework Directive.Current guidelines neglect the potential environmental impact of this drug, recommending that the cream should be applied head to toe and “washed off after 8–12 h”.Recently, a wiping procedure before hand washing after application of the topical treatment resulted in a 66 % reduction of the amount of diclofenac released in wastewater. The authors suggested that this method could be explored for other topical treatments. In the case of scabiosis, a protocol implicating the whole body needs to be designed.The absorption of topical permethrin is low. Considering the growing incidence of scabies, the amount of the pyrethroid reaching the environment may also be increasing. Therefore, we believe that applying the wiping procedure to the case of topical permethrin deserves consideration. Other measures to minimize the amount of permethrin residues reaching wastewater by washing clothes and bed linen such as wearing single-use pijamas should also be explored.In conclusion, we need to apply a One Health approach in the treatment with scabies, without neglecting the environmental impact of pharmaceuticals. It is not rational to forget drugs once they go down the drain.

Study on the pyrolysis characteristics and char gasification kinetics of pre-separated automobile shredder residues

Automobile shredder residue (ASR) is a typical solid waste produced by the end-of-life automobile industry. The pyrolysis and gasification of ASR are effective methods to achieve reduction and resource utilization. By means of thermogravimetric analysis and fixed-bed experiments, the distribution as well as physicochemical properties of five typical ASR pyrolysis products after pre-sorting were systematically investigated, and the gasification kinetics of their pyrolysis char under CO2 as well as steam were also studied. Results showed that the pyrolysis of ASR was basically completed at 500°C, and the pyrolysis gas yield increased significantly with temperature. The tar contained mainly poly-benzene rings with carbon atoms C11–15 and their derivatives. At high temperatures, the structure of pyrolysis char tended to become more ordered, with small functional group such as - OH, - NH, - CH3, - CC -, and - COOH fracturing. The residual amounts of heavy metals such as Cr, Pb, and Ni in pyrolysis char were mostly exceed 45%, which were significantly affected by the type of heavy metals, pyrolysis temperature and material type. Hydrogen production from steam gasification accounted for up to 65%, and the gasification reactivity was better than that of CO2 gasification. The activation energy (E) calculated through shrinking core model (SCM) and random pore model (RPM) was 38.81–105.44 kJ/mol and 39.53–98.56 kJ/mol, respectively, which provided a good fit to the gasification kinetics in two gasification atmospheres. This study provides detailed data for the ASR pyrolysis and gasification technology, which is an important reference value for ASR research.